Antimicrobial efficacy of Casuarina equisetifolia extracts against some pathogenic microorganisms

Dynamic Chiropractic

关键词: Casuarina equisetifolia;    antibacterial;    antifungal;    phytochemical constituents;    crude extract;   

DOI  :  10.5897/JMPR12.741

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Casuarina equisetifolia(Casuarinaceae) is a plant that is used in folk medicine for the treatment of astringent, in diarrhea, cough, ulcers, toothache, lotion for swelling and diabetes. To evaluate the scientific basis for the use of the plant, the antimicrobial activities of leaves extract was investigated against 7 medically important bacterial strains, namelyBacillus subtilis, methicillin-resistantStaphylococcusaureus(MRSA),Micrococcus,Escherichia coli,Pseudomonas aeruginosaandKlebsiella pneumoniaeand 4 fungi. The antibacterial activity of aqueous and organic solvents was determined by agar well diffusion method. The most pronounced effect was shown by the methanol extract. The most susceptible bacteria wereS. aureus,followed byK. pneumoniae,while the most resistant bacteria wasB. subtilisfollowed byMicrococcus. The antifungal activity of aqueous and organic solvents was also determining. The most pronounced effect was shown by ethanolic. The most susceptible fungi wereAspergillusflavuswhile the most resistant fungi wereCandida albicans. The high performance liquid chromatography (HPLC) analysisindicated the presence of 8 phenolic compounds as major active constituent (gallic, protocatioic, chlorogenic, syringic, p.hydroxy benzoic, p.coumaric, vanillic and salicylic acid). Result obtained indicated variable difference in concentration of the compounds inC. equisetifolialeaves extract. Results of HPLC showed gallic, salicylic and protocatioic in high concentration of 19.18, 11.57 and 6.84 µg/g, respectively. The concentration of other phenolic compounds ranged from 1.63 to 4.70 µg/g. The least concentration was chlorogenic with 1.63 µg/g.

【 授权许可】

Unknown