Validation of methodology for the chemical characterization of sugar cane bagasse

Quimica nova

Nascimento, Renata Trajano do¹  Gouveia, Ester Ribeiro¹  Souto-Maior, Ana Maria¹  Rocha, George Jackson de Moraes² 

[1] Universidade Federal de Pernambuco, Recife, Brasil;Universidade de São Paulo, Lorena, Brasil

关键词: bagasse;    HPLC;    validation.;   

DOI  :  10.1590/S0100-40422009000600026

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

In this work, amethodology for the characterization of sugar cane bagasse was validated. Bagassepre-treated with steam in a 5000 L reactor at a pressure of 15.3 kgf/cm2,during 7 min, was used to test the methodology. The methodology consisted ofthe hydrolysis of the material with H2SO4 at 72% v/v,for the quantification of carbohydrates, organic acid, furfural and hydroxymethylfurfuralby HPLC; insoluble lignin and ash by gravimetry; and soluble lignin by spectrophotometry.Linearity, repeatability, reproducibility and accuracy of the results obtainedin two Research Laboratories were determined, and were considered to be suitablefor the validation of the methodology.

【 授权许可】

CC BY