Validation of methodology for the chemical characterization of sugar cane bagasse

Química Nova

Nascimento, Renata Trajano do¹  Rocha, George Jackson de Moraes¹  Gouveia, Ester Ribeiro¹  Universidade Federal de Pernambuco, Recife, Brasil¹  Souto-Maior, Ana Maria¹  Universidade de São Paulo, Lorena, Brasil¹ 

关键词: bagasse;    HPLC;    validation.;   

DOI  :  10.1590/S0100-40422009000600026

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

In this work, a methodology for the characterization of sugar cane bagasse was validated. Bagasse pre-treated with steam in a 5000 L reactor at a pressure of 15.3 kgf/cm2, during 7 min, was used to test the methodology. The methodology consisted of the hydrolysis of the material with H2SO4 at 72% v/v, for the quantification of carbohydrates, organic acid, furfural and hydroxymethylfurfural by HPLC; insoluble lignin and ash by gravimetry; and soluble lignin by spectrophotometry. Linearity, repeatability, reproducibility and accuracy of the results obtained in two Research Laboratories were determined, and were considered to be suitable for the validation of the methodology.

【 授权许可】

Unknown