Comparative analysis of the venom proteome of four important Malaysian snake species

Journal of venomous animals and toxins

Jaya Vejayan¹  Too Lay Khoon²  Halijah Ibrahim² 

[1] Faculty of Industrial Sciences and Technology, University Malaysia Pahang, Kuantan, Malaysia;Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia

关键词: Mass spectrometry;    Snake venoms;    Proteomics;    Two-dimensional gel electrophoresis;   

DOI  :  10.1186/1678-9199-20-6

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Naja kaouthia, Ophiophagus hannah, Bungarus fasciatus and Calloselasma rhodostoma are four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared. The 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma – 26, N. kaouthia – 16, O. hannah – 15 and B. fasciatus – 6. Despite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified included phospholipase A2 proteins in all four venoms, long neurotoxins in both cobra species and the C. rhodostoma venom found with the most varied types of peptidases, i.e. metalloproteinase kistomin, halystase and L-amino acid oxidase.

【 授权许可】

CC BY