Low concentrations of doxycycline attenuates FasL-induced apoptosis in HeLa cells

Biological research: BR

Sun Jin Hur¹  Sushruta Koppula²  Jung Mi Yoon²  Chan Gil Kim²  Se Jong Huh² 

[1] Department of Animal Science and Technology, Chung-Ang University, Anseong, South Korea;Department of Biotechnology, Konkuk University, Chungju, Republic of Korea

关键词: Apoptosis;    Caspase;    Cisplatin;    FasL;    Hydrogen peroxide;    Tetracycline;   

DOI  :  10.1186/s40659-015-0025-8

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Doxycycline (DC) has been shown to possess non-antibiotic properties including Fas/Fas Ligand (FasL)-mediated apoptosis against several tumor types in the concentration range of 10–40 µg/mL. However, the effect of DC in apoptotic signaling at much low concentrations was not studied. The present study investigated the attenuation effect of low dose of DC on FasL-induced apoptosis in HeLa cell by the methods of MTT assay, fluorescence microscopy, DNA fragmentation, flow cytometry analysis, and western blotting. In the present findings we showed that low concentration of DC (

【 授权许可】

CC BY