Islet Culture/Preservation before Islet Transplantation

Cell Medicine

Masayuki Matsushita¹  Chika Miyagi-Shiohira²  Kiyoto Kurima²  Hirofumi Noguchi²  Masami Watanabe³  Yasufumi Noguchi⁴  Naoya Kobayashi⁵  Issei Saitoh⁶ 

[1] # Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan;* Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan;§ Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;¶ Department of Socio-environmental Design, Hiroshima International University, Hiroshima, Japan;† Okayama Saidaiji Hospital, Okayama, Japan;‡ Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan

关键词: Islet transplantation;    Culture condition;    Preservation solution;    University of Wisconsin (UW) solution;    Low temperature;   

DOI  :  10.3727/215517915X689047

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Although islet culture prior to transplantation provides flexibility for the evaluation of isolated islets and the pretreatment of patients, it is well known that isolated islets deteriorate rapidly in culture. Human serum albumin (HSA) is used for medium supplementation instead of fetal bovine serum (FBS), which is typically used for islet culture research, to avoid the introduction of xenogeneic materials. However, FBS contains several factors that are beneficial to islet viability and which also neutralize the endogenous pancreatic enzymes or exogenous enzymes left over from the isolation process. Several groups have reported the comparison of cultures at 22°C and 37°C. Recent studies have demonstrated the superiority of 4°C preservation to 22°C and 37°C cultures. We herein review the current research on islet culture/preservation for clinical islet transplantation.

【 授权许可】

CC BY