Concentration and Chain Length of Polyethylene Glycol in Islet Isolation Solution: Evaluation in a Pancreatic Islet Transplantation Model

Cell Transplantation

Article

B. Barrou¹  T. Hauet²  M. Eugene²  R. Thuillier²  S. Giraud²  D. Bon³  Y. Neuzillet⁴ 

[1] INSERM U1082, Poitiers, France;Service d'Urologie, GH Pitié-Salpêtrière, AP-HP, Paris, France;UPMC Université Paris VI, Paris, France;INSERM U1082, Poitiers, France;Service de Biochimie, CHU Poitiers, Poitiers, France;Université de Poitiers, Faculté de Médicine et de Pharmacie, Poitiers, France;INSERM U1082, Poitiers, France;Université de Poitiers, Faculté de Médicine et de Pharmacie, Poitiers, France;Service d'urologie et transplantation rénale, Hôpital Foch, Suresnes, France;Faculté de médecine Paris-Ile de France ouest, UVSQ, Versailles, France;

关键词: Ischemia reperfusion injury;    Preservation solution;    Polyethelene glycol (PEG);    Graft preservation;    Graft outcome;   

DOI  :  10.3727/096368912X638928

 received in 2010-11-03, accepted in 2011-11-18,  发布年份 2012

来源: Sage Journals

【 摘 要 】

To improve graft preservation and consequently reduce conservation injuries, the composition of preservation solution is of outmost importance. It was demonstrated that the colloid polyethylene glycol (PEG), used in SCOT solution, has protective effects on cell membranes and immunocamouflage properties. The aim of this study was to optimize the concentration and chain length of PEG to improve pancreatic islet preservation and outcome. In a model of murine islet allotransplantation, islets were isolated with SCOT containing various concentrations of PEG 20 kDa or 35 kDa. Better islet yield (IEQ) was obtained with SCOT + PEG at 15–30 g/L versus other PEG concentrations and control CMRL-1066 + 1% BSA solution (p < 0.05). Allograft survival was better prolonged (up to 20 days) in the groups SCOT + PEG 20 kDa 10–30 g/L compared to PEG 35 kDa (less than 17.8 days) and to control solutions (less than 17.5 days). In terms of graft function recovery, the use of PEG 20 kDa 15–30 g/L induced no primary nonfunction and delayed graft function contrary to CMRL-1066 and other PEG solutions. The use of the extracellular-type solution SCOT containing PEG 20 kDa 15 g/L as colloid could be a new way to optimize graft integrity preservation and allograft outcome.

【 授权许可】

Unknown   
© 2012 Cognizant Comm. Corp.

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