Comparison of Incubation Solutions Prior to the Purification of Porcine Islet Cells

Cell Medicine

Yasufumi Noguchi¹  Masami Watanabe²  Takashi Kawai³  Takashi Kuise³  Noriko Imagawa³  Toshiyoshi Fujiwara³  Hitomi Usui Kataoka⁴  Issei Saitoh⁵  Hirofumi Noguchi⁶  Akihiro Katayama⁷  Atsuko Nakatsuka⁷ 

[1] #Department of Socio-environmental Design, Hiroshima International University, Hiroshima, Japan;**Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;*Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;§Department of Primary Care and Medical Education, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;¶Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan;†Department of Surgery, Chiba-East National Hospital, National Hospital Organization, Chiba, Japan;‡Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

关键词: Islet transplantation;    Islet isolation;    University of Wisconsin (UW) solution;    HN-1 solution;    Preincubation;   

DOI  :  10.3727/215517913X674180

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

For pancreatic islet transplantation, one of the most important steps of islet isolation is islet purification. The most common method of islet purification is density gradient centrifugation because there are differences in density between islets and acinar tissue. However, the density of islets/acinar tissue depends on several conditions, such as the incubation time before purification and the osmolality of the preincubation solution. In this study, we evaluated the impact of using two different preincubation solutions before purification. We used the University of Wisconsin (UW) solution and a new preservation solution (HN-1), which we recently developed. There were no significant differences between the two solutions in terms of the islet yield, rate of viability, and purity or stimulation index after purification. There were also no differences in the attainability and suitability of posttransplantation normoglycemia. Our study shows that the HN-1 solution is equivalent to the UW solution for preincubation before islet purification.

【 授权许可】

CC BY