Comparison of New Preservation Solutions, HN-1 and University of Wisconsin Solution, in Pancreas Preservation for Porcine Islet Isolation

Cell Medicine

Yasufumi Noguchi¹  Masami Watanabe²  Akihiro Katayama³  Daisho Hirota³  Atsuko Nakatsuka³  Jun Wada³  Kentaro Inoue³  Hitomi Usui Kataoka⁴  Issei Saitoh⁵  Hirofumi Noguchi⁶  Noriko Imagawa⁷  Toshiyoshi Fujiwara⁷  Takashi Kawai⁷  Takashi Kuise⁷ 

[1] #Department of Socio-environmental Design, Hiroshima International University, Hiroshima, Japan;**Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;*Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan;§Department of Primary Care and Medical Education, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;¶Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan;†Department of Surgery, Chiba-East National Hospital, National Hospital Organization, Chiba 260-8712, Japan;‡Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

关键词: Islet transplantation;    Islet isolation;    HN-1 solution;    University of Wisconsin (UW) solution;    Preservation;   

DOI  :  10.3727/215517913X674171

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

For pancreatic islet transplantation, maintaining organ viability after pancreas procurement is critical and a major determinant for better graft function and survival. University of Wisconsin (UW) solution is currently the gold standard for abdominal organ preservation and the pancreas in particular. However, in the use of UW preservation solution for islet transplantation, there are disadvantages to be overcome, such as the inhibition of collagenase activity during pancreatic digestion. In this study, we compared UW solution with HN-1 solution in pancreas preservation for islet isolation. Islet yield was significantly greater in the HN-1 group than the UW group both before and after purification. In the in vitro assay, the adenosine triphosphate content in cultured islets was significantly higher in the HN-1 group than in the UW group. Furthermore, in streptozotocin-induced diabetic nude mice, the islet graft function of the HN-1 group was superior to that of the UW group. We concluded that the use of HN-1 solution is a promising approach for optimal pancreas preservation in islet transplantation.

【 授权许可】

CC BY