MAIT Cells Detect and Efficiently Lyse Bacterially-Infected Epithelial Cells

PLoS Pathogens

Nacer-Eddine Serriari¹  Emmanuel Treiner¹  Marie-Lise Gougeon²  Philippe Sansonetti³  Olivier Lantz⁴  Emmanuel Martin⁴  Virginie Premel⁴  Armelle Bohineust⁴  David Sleurs⁴  Claire Hivroz⁴  Maxime Coré⁴  Lionel Le Bourhis⁴  Claire Soudais⁴  Mathilde Dusseaux⁴  Stéphanie Bessoles⁴ 

[1] Avenir Group Inserm UMR925, Amiens, France;Institut Pasteur, Unité Immunité Antivirale, Biothérapies et Vaccins, Paris, France;Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, U786, Paris, France;Institut curie, Inserm U932, Paris, France

关键词: T cells;    Epithelial cells;    HeLa cells;    Shigellosis;    Bacterial pathogens;    Salmonellosis;    Cytokines;    Shigella;   

DOI  :  10.1371/journal.ppat.1003681

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Mucosal associated invariant T cells (MAIT) are innate T lymphocytes that detect a large variety of bacteria and yeasts. This recognition depends on the detection of microbial compounds presented by the evolutionarily conserved major-histocompatibility-complex (MHC) class I molecule, MR1. Here we show that MAIT cells display cytotoxic activity towards MR1 overexpressing non-hematopoietic cells cocultured with bacteria. The NK receptor, CD161, highly expressed by MAIT cells, modulated the cytokine but not the cytotoxic response triggered by bacteria infected cells. MAIT cells are also activated by and kill epithelial cells expressing endogenous levels of MRI after infection with the invasive bacteria Shigella flexneri. In contrast, MAIT cells were not activated by epithelial cells infected by Salmonella enterica Typhimurium. Finally, MAIT cells are activated in human volunteers receiving an attenuated strain of Shigella dysenteriae-1 tested as a potential vaccine. Thus, in humans, MAIT cells are the most abundant T cell subset able to detect and kill bacteria infected cells.

【 授权许可】

CC BY