LPS stimulates IgM production in vivo without help from non-B cells:

Innate Immunity

MingfangLu¹ 

关键词: B cells;    Innate Ab;    Lipopolysaccharide;    Lymph node;    Toll-like receptor 4;   

DOI  :  10.1177/1753425916644675

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Gram-negative bacterial LPS induce murine B-cell activation and innate (polyclonal) Ab production. Mouse B cells express the LPS signaling receptor (TLR4), yet how LPS activates B-cell responses in vivo is not known. Can LPS directly stimulate B cells to induce innate Ab production? Is activation of non-B cells also required? To address these questions, we transfused LPS-responsive (Tlr4+/+) or non-responsive (Tlr4−/−) B cells into LPS-responsive or non-responsive mice. Increased expression of the early activation markers CD69 and CD86 could be induced on transfused Tlr4−/− B cells by injecting LPS subcutaneously into Tlr4+/+ mice, demonstrating indirect activation of B cells by TLR4-responsive non-B cells in vivo, but the Tlr4−/− B cells did not increase serum IgM levels. In contrast, when Tlr4−/− recipients were transfused with Tlr4+/+ B cells, LPS induced large amounts of serum IgM and LPS could also enhance specific Ab production to a protein that was co-injected with it (adjuvant response). Thus, LPS...

【 授权许可】

CC BY