THE EFFECT OF CRUDE PROTEIN HALIMEDA SP. ON CYPRINUS CARPIO INFECTED KOI HERPES VIRUS ON EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-1

JFMR-Journal of Fisheries and Marine Research

Uun Yanuhar¹  Rika Wahyuningtyas¹ 

[1] Brawijaya University

关键词: Cyprinus carpio;    Halimeda;    KHV;    protein;    MHC-I;   

DOI  :  10.21776/ub.jfmr.2017.001.01.3

学科分类：农业科学（综合）

来源: University of Brawijaya

【 摘 要 】

Halimeda sp. is a kind species of macroalgae that abundant grow in Indonesia. The utilization of Halimeda especially protein for disease prevention caused by the virus has not been done to the fish. The purpose of the study is to know the  treatment of crude protein Halimeda sp on   Cyprinus carpioinfected by Koi Harvest Virus (KHV) on the expression of Major Histocompatibility Complex (MHC) class 1. MHC class-1 is one of the immune adaptive response on fish like this C. carpio. The methods are isolation of crude protein Halimeda sp,   purification  of  crude protein, Haemagglutination (HA) test and dot blot test. The result shows that the crude protein ofHalimedasp. can produce of adaptive immune response like MHC class 1. MHC class-1 has the function of  the immune system for maintenance of virus attacks directly. Responsible of treatment crude protein Halimeda sp. onC. carpiocan be showed quantitatively using the dot blot test result. The  conclusion  is the crude protein of Halimeda sp. able to activate the MHC class I and to inhibit the proliferation of the KHV and maintenance of the fish cell of C.carpio ..

【 授权许可】

CC BY-NC