Isolation and identification of bacteria with antimicrobial activities from green algae (Halimeda discoidea) from Pulau Lima, Banten Bay, Indonesia

3rd EMBRIO International Workshop on Marine Biodiversity: Understanding, Utilization, Conservation

生态环境科学

Bawanti, N.P.S.C.^1 ; Siregar, R.R.^1 ; Kristianty, M.G.E.^2 ; Indriati, N.^3

Department of Fish Processing Technology, Jakarta Fisheries University, Jakarta, Indonesia^1

Department of Aquaculture Technology, Jakarta Fisheries University, Jakarta, Indonesia^2

Research and Development Center for Marine and Fisheries Processing and Biotechnology, Jakarta, Indonesia^3

关键词: Anti-bacterial activity;    Anti-microbial activity;    Anti-microbial properties;    Gram-positive bacterium;    Halimeda;    Isolation and identification;    Minimum inhibitory concentration;    Pseudomonas guezennei;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/278/1/012010/pdf
DOI  :  10.1088/1755-1315/278/1/012010

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Bacteria associated with seaweed were involved in the production of metabolites associated with their host, so they tend to have nearly the same metabolites to defend themselves or as antibiotics. The aim of this study was to isolate and to identify bacteria with antimicrobial activities from the marine green algae (Halimeda discoidea). Laboratory analysis was for the isolation of bacteria, selection of bacteria with antimicrobial activities, antibacterial activity tests and Minimum Inhibitory Concentration (MIC). Results showed that six bacterial isolates were isolated from outside and inside of the algae, which have antimicrobial activities. One was selected for further work which was have more antimicrobial activities. The bacteria showed antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus) but not against Gram-negative bacteria (Escherichia coli). The bacterial growth curve was at an optimum phase at the 10th to 16th hour. Microscopic analysis and biochemical tests showed that isolated bacteria were a rod-shaped Gram-negative, acid-free, non-endosporous, reacted positively to gelatin, citrate and carbohydrate, reacting negatively on motile, and urease. We conclude that the bacteria isolated from green algae which had more antimicrobial properties belongs to the genus Pseudomonas guezennei.