期刊论文详细信息
Stem Cell Research & Therapy
Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells
Susanna Miettinen2  Bettina Mannerström2  Mohan C Vemuri1  Andrew Campbell3  Shayne Boucher1  Miia Juntunen2  Mimmi Patrikoski2 
[1] Life Technologies, Cell Therapy Systems, Frederick, MD, USA;Science Center, Tampere University Hospital, Tampere, Finland;Life Technologies, BioProduction, Grand Island, MD, USA
关键词: Cell therapy;    Flow cytometry;    Immunophenotype;    Proliferation rate;    Multipotentiality;    Fetal bovine serum;    Human serum;    Serum-free;    Xeno-free;    Adipose stem cells;   
Others  :  848436
DOI  :  10.1186/scrt175
 received in 2012-09-21, accepted in 2013-03-04,  发布年份 2013
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【 摘 要 】

Introduction

Adipose tissue is an attractive and abundant source of multipotent stem cells. Human adipose stem cells (ASCs) have shown to have therapeutic relevancy in diverse clinical applications. Nevertheless, expansion of ASCs is often necessary before performing clinical studies. Standard in vitro cell-culture techniques use animal-derived reagents that should be avoided in clinical use because of safety issues. Therefore, xeno- and serum-free (XF/SF) reagents are highly desirable for enhancing the safety and quality of the transplanted ASCs.

Methods

In the current study, animal component-free isolation and cell-expansion protocols were developed for ASCs. StemPro MSC SFM XF medium with either CELLstart™ CTS™ coating or Coating Matrix Kit were tested for their ability to support XF/SF growth. Basic stem-cell characteristics such as immunophenotype (CD3, CD11a, CD14, CD19, CD34, CD45RO, CD54, CD73, CD80, CD86, CD90, CD105, HLA-DR), proliferation, and differentiation potential were assessed in XF/SF conditions and compared with human serum (HS) or traditionally used fetal bovine serum (FBS) cultures.

Results

ASCs cultured in XF/SF conditions had significantly higher proliferation rates compared with HS/FBS cultures. Characteristic immunophenotypes of ASCs were maintained in every condition; however, cells expanded in XF/SF conditions showed significantly lower expression of CD54 (intercellular adhesion molecule 1, ICAM-1) at low passage number. Further, multilineage differentiation potential of ASCs was maintained in every culture condition.

Conclusions

Our findings demonstrated that the novel XF/SF conditions maintained the basic stem cell features of ASCs and the animal-free workflow followed in this study has great potential in clinical cell therapies.

【 授权许可】

   
2013 Patrikoski et al.; licensee BioMed Central Ltd.

【 预 览 】
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