Effects of Sucrose and Browning Inhibitors on Callus Proliferation and Anti-Browning of Chinese Kale

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Liang, Sha^1 ; He, Ying^1 ; Zheng, Hao^1 ; Yuan, Qiao^1 ; Zhang, Fen^1 ; Sun, Bo^1

College of Horticulture, Sichuan Agricultural University, Chengdu

611130, China^1

关键词: Chinese kales;    Culture medium;    Genetic improvements;    Poly vinyl pyrrolidone;    Proliferation rate;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/2/022018/pdf
DOI  :  10.1088/1755-1315/252/2/022018

来源: IOP

【 摘 要 】

The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants, the effects of the different concentrations of sucrose and different browning inhibitors (activated carbon, polyvinylpyrrolidone, and ascorbic acid) on the callus proliferation and anti-browning of Chinese kale were studied in this study. The results showed that the proliferation medium with 20 g•L-1 sucrose had the best effects on the proliferation and anti-browning of Chinese kale callus, and its proliferation rate was the highest, reaching 213.5%, and the browning rate was as low as 27.77%. In addition, adding 0.2 g•L-1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate. In this treatment, the callus had the lowest browning rate of 19.45% and the highest proliferation rate of 266.98%, and the morphology of the callus was friable. This study lays a foundation for future research in molecular biology and genetic improvement in Chinese kale.