Thidiazuron amends the organ development of endangered aquatic plant Cryptocoryne elliptica Hook. F

International Fundamentum Science Symposium 2018

自然科学;工业技术

Norhanizan, S.^1,2 ; Aziz, A.^2

Fisheries Research Institute, Freshwater Fisheries Research Division, Glami Lemi, Titi, Jelebu, Negeri Sembilan

71650, Malaysia^1

School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu

21030, Malaysia^2

关键词: Endangered species;    Micropropagation;    Multiple shoots;    Murashige and skoog;    Organ development;    Proliferation rate;    Protocorm-like body;    Randomized design;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/440/1/012046/pdf
DOI  :  10.1088/1757-899X/440/1/012046

来源: IOP

【 摘 要 】

Cryptocoryne elliptica is a Malaysia endogenous aquatic plants that has been categorized as endangered species. The plant received a highly demand for aquarium trade and being collected from wild. Slow growth and propagates only through runners has obstructs the plantlets production. Micro propagation is the best option. However, study on the effects of cytokinin particularly thidiazuron (TDZ) on the proliferation rate of this plant in vitro is still limited. Thus, the objective of this study was to determine the effects of thidiazuron on multiple shoots proliferation under in vitro conditions. A complete randomized design was used. TDZ was tested at the concentrations of 0.2 - 1.0 mg/l in two media: Murashige and Skoog (MS) and Linsmaier and Skoog (LS), respectively. Single shoot explant was used for each culture vessel with thirty cultures per-treatment. Results showed that the highest shoots number generated per explant was obtained in MS + 0.8 mg/l TDZ, which was 7.33 3.08 after 8 weeks of cultures. The protocorm-like-bodies (PLBs) were produced in MS + 0.4 - 0.8 mg/l TDZ and LS + 0.2 - 1.0 mg/l TDZ, after 28 weeks of culture, respectively. The inflorescences were also formed on the explants cultured on TDZ containing medium. This finding suggested that TDZ had alters the proliferation and differentiation on C. elliptica cells.