In vitro micropropagation of Raja Bulu banana in medium supplemented with coconut water and NAA

International Conference on Sustainable Agriculture for Rural Development 2018

Ajie, W.K.B.^1 ; Malinda, R.^1 ; Yuniastuti, E.^1 ; Yunus, A.^1

Agrotechnology, Agriculture Faculty, University of Sebelas Maret, Central Java, Surakarta, Indonesia^1

关键词: Coconut water;    Completely randomized designs;    Increasing production;    Indonesia;    Micropropagation;    Plant physiology;    Root formation;    Tropical fruits;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/250/1/012014/pdf
DOI  :  10.1088/1755-1315/250/1/012014

来源: IOP

【 摘 要 】

Raja Bulu banana is one of popular tropical fruit in Indonesia. Increasing production of Raja Bulu banana can be done by in vitro culture with addition coconut water and NAA. This research was conducted in April-November 2017 at Plant Physiology and Biotechnology Laboratory Faculty of Agriculture, Sebelas Maret University. The experimental design was Completely Randomized Design (CRD) two factors: coconut water (K) with concentration K1 = 50 ml l-1, K2 = 100 ml l-1, K3 = 150 ml l-1, and NAA (A) with concentration A0 = 0 ppm; A1 = 0.5 ppm; A2 = 1 ppm; A3 = 1.5 ppm. The result showed that combination of coconut water at 50 ml l-1 and NAA 0,5 ppm resulted in highest number of shoots (5.6 shoots). Medium supplemented with coconut water 100 ml l-1 and NAA 0 ppm resulted in highest number of leaves (8 leaves). In the medium treated with NAA 1.5 ppm, shoots appeared after six days of planting and yielded the highest number of roots (11.55 roots). Coconut water increased the shoot height at concentrations of 50 ml l-1, accelerated root formation at concentrations of 100 ml l-1, and increased number of roots at concentrations of 50 ml l-1.