The modification of murashige and skoog media for efficient cultivation of gizella-5 and vsl-2 rootstocks in vitro

International Scientific Conference «AGRITECH-2019: Agribusiness, Environmental Engineering and Biotechnologies»

农业科学;生态环境科学;生物科学

Bamatov, Ibragim^1 ; Butsaeva, Elina^1 ; Arsanov, Magomed^1 ; Bamatov, Dzhamalai^1

Chechen State University, 17 Dudaev Boulevard ave., Grozny, Chechen

366007, Russia^1

关键词: 6-benzylaminopurine;    Experimental control;    In-vitro;    Murashige and skoog;    Murashige and Skoog mediums;    Nutrient media;    Regeneration process;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/315/4/042015/pdf
DOI  :  10.1088/1755-1315/315/4/042015

来源: IOP

【 摘 要 】

This paper reports the effect of modified nutrient media on biotechnologically growing rootstock plants (Gizella - 5 and VSL - 2) at the stages of introduction and grafting. Murashige and Skoog medium was modified by adding 25 mg/l Chelate-Fe and 500 mg/l of Ca(NO)3 to the basic nutrient at the stage of introduction of the plant. At the grafting stage 0.1 mg of 6-Benzylaminopurine (6-BAP) was added to Murashige and Skoog, where the effect of regeneration was 2 times higher than in experimental control. The results obtained prove that modified Murashige and Skoog medium is to be the best nutrient media for root-plant introduction and regeneration processes in Vitro followed by Chelate-Fe, Ca(NO)3 and 6-BAP respectively.