Preparation of Degradable Biological Carrier With LCC and its Application in Culture of Hepatocytes

International Conference on Computer Information and Automation Engineering

计算机科学;运输工程

Zhao, H.K.^1 ; Chen, X.K.^1 ; Wu, H.F.^1 ; Li, J.L.^1 ; Xie, Y.M.^1,2

Research Institute of Pulp and Paper Engineering, Hubei University of Technology, Wuhan, China^1

Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, Wuhan, China^2

关键词: Biological carriers;    Control groups;    Freeze drying method;    FT-IR spectrum;    Hepatocyte culture;    ITS applications;    Lignin-carbohydrate complex;    Proliferation rate;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/359/1/012036/pdf
DOI  :  10.1088/1757-899X/359/1/012036

学科分类：计算机科学（综合）

来源: IOP

【 摘 要 】

The purpose of this article is to extract lignin-carbohydrate complexes (LCC) with poplar as raw material, which was used to prepare bio-carrier by freeze-drying method. The chemical properties and morphological of LCC porous biological carriers were analyzed by GPC, FT-IR, scanning electron microscopy (SEM) and optical microscopy. The FT-IR spectrum results indicated that LCC which are composed of lignin and polysaccharide, with a typical LCC structure. Galactose have a specific ability to recognize liver cells owing to the presence of receptors on hepatocytes. Cell counting results showed that the cells increases fastest while the proliferation rate of the liver cell in LCC is obviously higher than that of control group. These results indicated that poplar LCC is very biocompatible, in which it might be a great potential biological carrier material for human hepatocyte culture.