Proteomic analysis of seminal fluid from men exhibiting oxidative stress

Reproductive Biology and Endocrinology

Edmund Sabanegh³  Satya P Yadav⁵  Belinda Willard⁴  Banu Gopalan²  Stefan S Du Plessis⁶  Gayatri Mohanty¹  Ashok Agarwal³  Rakesh Sharma³ 

[1] Permanent address: Ravenshaw University, Cuttack, Odisha, India;Bioinformatics Core Services, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA;Proteomics Core Services, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;Molecular Biotechnology Core lab, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;Medical Physiology, Stellenbosch University, Tygerberg, South Africa

关键词: Gene ontology;    Differentially expressed proteins;    Mass spectroscopy;    Proteomics;    Oxidative stress;    Reactive oxygen species;    Varicocele;    Male infertility;    Seminal plasma;   

Others  :  810017
DOI  :  10.1186/1477-7827-11-85

 received in 2013-06-18, accepted in 2013-08-28,  发布年份 2013

【 摘 要 】

Background

Seminal plasma serves as a natural reservoir of antioxidants. It helps to remove excessive formation of reactive oxygen species (ROS) and consequently, reduce oxidative stress. Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms underlying oxidative stress and sperm dysfunction in infertile men.

Methods

This prospective study consisted of 52 subjects: 32 infertile men and 20 healthy donors. Once semen and oxidative stress parameters were assessed (ROS, antioxidant concentration and DNA damage), the subjects were categorized into ROS positive (ROS+) or ROS negative (ROS-). Seminal plasma from each group was pooled and subjected to proteomics analysis. In-solution digestion and protein identification with liquid chromatography tandem mass spectrometry (LC-MS/MS), followed by bioinformatics analyses was used to identify and characterize potential biomarker proteins.

Results

A total of 14 proteins were identified in this analysis with 7 of these common and unique proteins were identified in both the ROS+ and ROS- groups through MASCOT and SEQUEST analyses, respectively. Prolactin-induced protein was found to be more abundantly present in men with increased levels of ROS. Gene ontology annotations showed extracellular distribution of proteins with a major role in antioxidative activity and regulatory processes.

Conclusions

We have identified proteins that help protect against oxidative stress and are uniquely present in the seminal plasma of the ROS- men. Men exhibiting high levels of ROS in their seminal ejaculate are likely to exhibit proteins that are either downregulated or oxidatively modified, and these could potentially contribute to male infertility.

【 授权许可】

   
2013 Sharma et al.; licensee BioMed Central Ltd.

【 图 表 】

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