Study of proteins after recovery from bacterial infection

Proteomics;tandem mass spectrometry (MS/MS);Bacillus Calmette–Guérin (BCG);Functional enrichment

Zavala, Cynthia ; Rodriguez-Zas ; Sandra L.

关键词: Proteomics;    tandem mass spectrometry (MS/MS);    Bacillus Calmette–Guérin (BCG);    Functional enrichment;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/72851/Cynthia_Zavala.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

The effect of Bacillus Calmette–Guérin (BCG) on the microglia of mice after recovery from infection is incompletely understood. Microglia cells of mice were collected from two groups, mice infected with BCG vs. control mice (BCG vs. control). The objective of this study was to compare the proteins and peptides present in the microglia of BCG-challenged mice one-week after treatment relative to control mice and gain insights through differential detection and enrichment analysis.Detection relied on tandem mass spectrometry proteomics. The database search software PEAKS was used to detect peptides and proteins in the treated and control samples. Differential detection was performed using SAS procedures and enrichment classification was completed using PANTHER. The consistently higher number of proteins and peptides (except for one sample) detected in the control samples suggested that BCG impacts the production of proteins. A number of proteins including F-actin-capping protein subunit alpha-2 (P47754), Alpha-enolase (P17182), and myelin basic protein (F7A0B0) were identified in greater abundance in control mice; while chitinase-like protein 3 (O35744), vesicle-associated membrane protein-associated protein A (Q9WV55), andProtein SET (Q9EQU5) were identified only in BCG-treated mice. The differential detection was consistent with similar studies on the neurological activity and inflammation response to BCG-challenged mice. Functional enrichment analysis identified enriched pathways among differentially detected proteins with a differential detection of two (BCG and control mice) associated with inflammation-mediated and microglia activation with Huntington disease (P00029) and Glycolysis (P00024).