Proteomic analysis of seminal fluid from men exhibiting oxidative stress

Reproductive Biology and Endocrinology

Research

Banu Gopalan¹  Edmund Sabanegh²  Rakesh Sharma²  Ashok Agarwal²  Gayatri Mohanty³  Stefan S Du Plessis⁴  Satya P Yadav⁵  Belinda Willard⁶ 

[1] Bioinformatics Core Services, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA;Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA;Permanent address: Ravenshaw University, Cuttack, Odisha, India;Medical Physiology, Stellenbosch University, Tygerberg, South Africa;Molecular Biotechnology Core lab, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;Proteomics Core Services, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;

关键词: Seminal plasma;    Male infertility;    Varicocele;    Reactive oxygen species;    Oxidative stress;    Proteomics;    Mass spectroscopy;    Differentially expressed proteins;    Gene ontology;   

DOI  :  10.1186/1477-7827-11-85

 received in 2013-06-18, accepted in 2013-08-28,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundSeminal plasma serves as a natural reservoir of antioxidants. It helps to remove excessive formation of reactive oxygen species (ROS) and consequently, reduce oxidative stress. Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms underlying oxidative stress and sperm dysfunction in infertile men.MethodsThis prospective study consisted of 52 subjects: 32 infertile men and 20 healthy donors. Once semen and oxidative stress parameters were assessed (ROS, antioxidant concentration and DNA damage), the subjects were categorized into ROS positive (ROS+) or ROS negative (ROS-). Seminal plasma from each group was pooled and subjected to proteomics analysis. In-solution digestion and protein identification with liquid chromatography tandem mass spectrometry (LC-MS/MS), followed by bioinformatics analyses was used to identify and characterize potential biomarker proteins.ResultsA total of 14 proteins were identified in this analysis with 7 of these common and unique proteins were identified in both the ROS+ and ROS- groups through MASCOT and SEQUEST analyses, respectively. Prolactin-induced protein was found to be more abundantly present in men with increased levels of ROS. Gene ontology annotations showed extracellular distribution of proteins with a major role in antioxidative activity and regulatory processes.ConclusionsWe have identified proteins that help protect against oxidative stress and are uniquely present in the seminal plasma of the ROS- men. Men exhibiting high levels of ROS in their seminal ejaculate are likely to exhibit proteins that are either downregulated or oxidatively modified, and these could potentially contribute to male infertility.

【 授权许可】

Unknown   
© Sharma et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.