The frequency of Y chromosome microdeletions in infertile men of Vellore cohort

14th International Conference on Science, Engineering and Technology

自然科学;工业技术

Ramgir, Shalaka S.^1 ; Sharma, Aditi^1 ; Kruyanshi, Master^1 ; Jaiswal, Anamika^1 ; Renu, Kaviyarasi^1 ; Prabhu, Yogamaya D.^1 ; Sekar, Nishu^1 ; Abilash, V.G.^1

Department of Biomedical Sciences, School of Bioscience and Technology, VIT Univeristy, Vellore, Tamilnadu

632014, India^1

关键词: Azoospermia;    Environmental factors;    Male infertility;    Sample sizes;    STS markers;    Y chromosomes;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/263/2/022033/pdf
DOI  :  10.1088/1757-899X/263/2/022033

来源: IOP

【 摘 要 】

Many male infertility cases are because of genetic and environmental factors and most of them are idiopathic. About 10-20% of azoospermic patients are showing the microdeletion in Y-chromosome. The azoospermia factor or AZF region at the Yq11 position which consists of genes those are necessary for spermatogenesis. In Y chromosome microdeletion, deletion in AZFa region is very rare. The aim of our study is to find out the frequency of microdeletions in Y chromosome particularly in AZFa region in azoospermic men of Vellore cohort. For this study, we collected 10 azoospermic patient and 10 control men samples from the Sandhya hospital, Vellore. In this study, we mainly focused on AZFa region to analyze the frequency of microdeletions in Y chromosome using SY82 (264bp) and SY83 (275bp) STS markers. There was an absence of microdeletion in patient samples for SY82 and SY83 markers of AZFa region. We concluded that sample size should be increased to confirm our results.