CHD1 and CHD2 are positive regulators of HIV-1 gene expression

Virology Journal

John AT Young²  Kenneth A Bradley¹  David J Banks¹  Melissa J Rodgers² 

[1] Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA;The Nomis Center for Immunobiology and Microbial Pathogenesis, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA

关键词: Transcription;    Chromatin remodeling;    CHD2;    CHD1;    HIV;   

Others  :  1148242
DOI  :  10.1186/1743-422X-11-180

 received in 2014-05-23, accepted in 2014-09-24,  发布年份 2014

【 摘 要 】

Background

Retroviruses encode a very limited number of proteins and therefore must exploit a wide variety of host proteins for completion of their lifecycle.

Methods

We performed an insertional mutagenesis screen to identify novel cellular regulators of retroviral replication.

Results

This approach identified the ATP-dependent chromatin remodeler, chromodomain helicase DNA-binding protein 2 (CHD2), as well as the highly related CHD1 protein, as positive regulators of both MLV and HIV-1 replication in rodent and human cells. RNAi knockdown of either CHD2 or the related CHD1 protein, in human cells resulted in a block to infection by HIV-1, specifically at the level of transcription.

Conclusions

These results demonstrate that CHD1 and CHD2 can act as positive regulators of HIV-1 gene expression.

【 授权许可】

   
2014 Rodgers et al.; licensee BioMed Central Ltd.

【 图 表 】

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