Transcription-coupled recruitment of human CHD1 and CHD2 influences chromatin accessibility and histone H3 and H3.3 occupancy at active chromatin regions

Epigenetics & Chromatin

Karl Ekwall¹  Andreas Lennartsson¹  Michelle Rönnerblad¹  Lina Cordeddu¹  Lee Siggens¹ 

[1] Department of Biosciences and Nutrition, NOVUM, Karolinska Institutet, Huddinge 141 83, Sweden

关键词: H3.3;    H3;    DNase;    ENCODE;    CHD2;    CHD1;    chromatin remodeling;   

Others  :  1114910
DOI  :  10.1186/1756-8935-8-4

 received in 2014-10-28, accepted in 2014-12-23,  发布年份 2015

【 摘 要 】

Background

CHD1 and CHD2 chromatin remodeling enzymes play important roles in development, cancer and differentiation. At a molecular level, the mechanisms are not fully understood but include transcriptional regulation, nucleosome organization and turnover.

Results

Here we show human CHD1 and CHD2 enzymes co-occupy active chromatin regions associated with transcription start sites (TSS), enhancer like regions and active tRNA genes. We demonstrate that their recruitment is transcription-coupled. CHD1 and CHD2 show distinct binding profiles across active TSS regions. Depletion of CHD1 influences chromatin accessibility at TSS and enhancer-like chromatin regions. CHD2 depletion causes increased histone H3 and reduced histone variant H3.3 occupancy.

Conclusions

We conclude that transcription-coupled recruitment of CHD1 and CHD2 occurs at transcribed gene TSSs and at intragenic and intergenic enhancer-like sites. The recruitment of CHD1 and CHD2 regulates the architecture of active chromatin regions through chromatin accessibility and nucleosome disassembly.

【 授权许可】

   
2015 Siggens et al.; licensee BioMed Central.

【 图 表 】

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