H3.3 kinetics predicts chromatin compaction status of parental genomes in early embryos

Reproductive Biology and Endocrinology

Shi-meng Guo¹  Xing-ping Liu¹  Li-quan Zhou¹ 

[1] Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, Hubei, China;

关键词: H3.3;    Chromatin mobility;    Totipotency;   

DOI  :  10.1186/s12958-021-00776-3

来源: Springer

【 摘 要 】

BackgroundAfter fertilization, the fusion of gametes results in the formation of totipotent zygote. During sperm-egg fusion, maternal factors participate in parental chromatin remodeling. H3.3 is a histone H3 variant that plays essential roles in mouse embryogenesis.MethodsHere, we used transgenic early embryos expressing H3.3-eGFP or H2B-mCherry to elucidate changes of histone mobility.ResultsWe used FRAP analysis to identify that maternally stored H3.3 has a more significant change than H2B during maternal-to-embryonic transition. We also found that H3.3 mobile fraction, which may be regulated by de novo H3.3 incorporation, reflects chromatin compaction of parental genomes in GV oocytes and early embryos.ConclusionsOur results show that H3.3 kinetics in GV oocytes and early embryos is highly correlated with chromatin compaction status of parental genomes, indicating critical roles of H3.3 in higher-order chromatin organization.

【 授权许可】

CC BY