Protective effects of Launaea procumbens on rat testis damage by CCl4

Lipids in Health and Disease

Rahmat Ali Khan¹ 

[1] Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology Bannu, Bannu, KPK, Pakistan

关键词: DNA damages;    Lipidperoxidation;    CCl₄;    GSH;    Launaea procumbens;   

Others  :  1160230
DOI  :  10.1186/1476-511X-11-103

 received in 2012-05-14, accepted in 2012-08-19,  发布年份 2012

【 摘 要 】

Background

Traditionally various human diseases of kidneys, hormonal imbalance and sexual diseases are treated with Launaea procumbens (L). In the present study protective effects of methanolic extract of Launaea procumbens (LPME) was evaluated against CCl₄-induced oxidative damages in rat testis.

Methods

To examine the protective effects of Launaea procumbens on testis against oxidative stress of carbon tetrachloride in male rat, 30 male albino rats were equally divided into 5 groups (6 rats). First group was given standard diet and drinking water. Second group received CCl₄ 3 ml/kg intraperitoneally (30% in olive oil). Third and forth were given orally 100; 200 mg/kg b.w., in 99.8% dimethyl sulphooxide (DMSO), Launaea procumbens methanolic extracts (LPME) after 48 h of CCl₄ treatment twice a week and sixth group received only LPME in DMSO at a dose of 200 mg/kg b.w., for four weeks. Protective effects of Launaea procumbens were observed on sperm concentration, motility and morphology, serum reproductive hormonal level, activity of antioxidant enzymes, lipid peroxidation (TBARS) and DNA damages.

Results

Results of the present study revealed that treatment of CCl₄ significantly (p < 0.01) reduced sperm concentration and motility comparatively to controls. Level of testosterone, luteinizing hormone and follicle stimulating hormone, were depleted markedly (p <0.01) with treatment of CCl₄. In addition, CCl₄ induction in rats reduced activities of antioxidant enzymes while increased lipid peroxidation and DNA damages. Co-administration of LPME significantly (p <0.01) improved these alterations in improving of hormonal level, activities of antioxidant enzymes and lipid peroxidation near to control rats.

Conclusion

From the results it is suggested that Launaea procumbens methanolic extract has the ability to protect testis against oxidative damages, possibly through antioxidant effects of its bioactive compounds.

【 授权许可】

   
2012 Khan; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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