Cardioprotective role of leaves extracts of Carissa opaca against CCl4 induced toxicity in rats

BMC Research Notes

Huda Mohammed Alkreathy¹  Rahmat Ali Khan⁴  Muhammad Rashid Khan²  Sumaira Sahreen³ 

[1] Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia;Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan;Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan;Department of Biotechnology, Faculty of Sciences, University of Science and Technology, Bannu, Pakistan

关键词: CCl₄;    Histopathalogical;    Lipid peroxidation;    DNA damages;   

Others  :  1133929
DOI  :  10.1186/1756-0500-7-224

 received in 2014-02-06, accepted in 2014-03-28,  发布年份 2014

【 摘 要 】

Background

Carissa opaca are used traditionally in Pakistan for the treatment of various human ailments. Therefore, the study is arranged out to assess the cardio protective potential of different fractions of Carissa opaca leaves on CCl₄-induced oxidative trauma in kidney.

Methods

The parameters studied in this respect were the cardiac function test (CK (U/l), CKMB (U/l), genotoxicity (% DNA fragmentation), characteristic morphological findings and antioxidant enzymatic level of cardiac tissue homogenate.

Result

The protective effects of various fractions of Carissa opaca (C. opaca) leaves extract against CCl₄ administration was reviewed by rat cardiac functions alterations. Chronic toxicity caused by eight week treatment of CCl₄ to the rats significantly changed the cardiac function test, decreased the activities of antioxidant enzymes and glutathione contents whereas significant increase was found in lipid peroxidation comparative to control group. Administration of various fractions of C. opaca leaves extract with CCl₄ showed protective ability against CCl₄ intoxication by restoring the cardiac functions alterations, activities of antioxidant enzymes and lipid peroxidation in rat. CCl₄ induction in rats also caused DNA fragmentation and histopathalogical abnormalities which were restored by co-admistration of various fraction of C. opaca leaves extract.

Conclusion

Results revealed that various fraction of C. opaca are helpful in cardiac dysfunctions.

【 授权许可】

   
2014 Sahreen et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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