3D Mapping of plasma effective areas via detection of cancer cell damage induced by atmospheric pressure plasma jets

27th Summer School and International Symposium on the Physics of Ionized Gases

Han, Xu^1,2 ; Liu, Yueing^3 ; Stack, M Sharon^3,4 ; Ptasinska, Sylwia^1,2,3

Radiation Laboratory, University of Notre Dame, Notre Dame

IN

46556, United States^1

Department of Physics, University of Notre Dame, Notre Dame

IN

46556, United States^2

Harper Cancer Research Institute, University of Notre Dame, Notre Dame

IN

46556, United States^3

Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame

IN

46556, United States^4

关键词: Atmospheric pressure plasma jets;    Cancer cells;    Damage level;    DNA damages;    Effective area;    Immunofluorescence assay;    Oral cancer cells;    Plasma treatment;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/565/1/012011/pdf
DOI  :  10.1088/1742-6596/565/1/012011

来源: IOP

【 摘 要 】

In the present study, a nitrogen atmospheric pressure plasma jet (APPJ) was used for irradiation of oral cancer cells. Since cancer cells are very susceptible to plasma treatment, they can be used as a tool for detection of APPJ-effective areas, which extended much further than the visible part of the APPJ. An immunofluorescence assay was used for DNA damage identification, visualization and quantification. Thus, the effective damage area and damage level were determined and plotted as 3D images.