Identification of pathways directly regulated by SHORT VEGETATIVE PHASE during vegetative and reproductive development in Arabidopsis

Genome Biology

Martin M Kater¹  George Coupland⁴  Lucia Colombo²  Giulio Pavesi¹  Paul E Grini³  Katrine N Bjerkan³  Gian Marco Prazzoli¹  Federico Zambelli¹  Stefano Torti⁴  Julieta L Mateos⁴  Sara Simonini¹  Rosalinda F Guerra¹  Alice Sessa¹  Fernando Andrés⁴  Veronica Gregis¹ 

[1] Department of Bioscience, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy;Consiglio Nazionale delle Ricerche Istituto di Biofisica, 20133 Milan, Italy;Department of Biosciences, University of Oslo, N-0316 Oslo, Norway;Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany

关键词: Arabidopsis thaliana;    floral development;    floral transition;    ChIP-seq;    post transcriptional regulation;    transcription factors;    gene regulation;    MADS-box;   

Others  :  866221
DOI  :  10.1186/gb-2013-14-6-r56

 received in 2013-03-24, accepted in 2013-06-11,  发布年份 2013

【 摘 要 】

Background

MADS-domain transcription factors play important roles during plant development. The Arabidopsis MADS-box gene SHORT VEGETATIVE PHASE (SVP) is a key regulator of two developmental phases. It functions as a repressor of the floral transition during the vegetative phase and later it contributes to the specification of floral meristems. How these distinct activities are conferred by a single transcription factor is unclear, but interactions with other MADS domain proteins which specify binding to different genomic regions is likely one mechanism.

Results

To compare the genome-wide DNA binding profile of SVP during vegetative and reproductive development we performed ChIP-seq analyses. These ChIP-seq data were combined with tiling array expression analysis, induction experiments and qRT-PCR to identify biologically relevant binding sites. In addition, we compared genome-wide target genes of SVP with those published for the MADS domain transcription factors FLC and AP1, which interact with SVP during the vegetative and reproductive phases, respectively.

Conclusions

Our analyses resulted in the identification of pathways that are regulated by SVP including those controlling meristem development during vegetative growth and flower development whereas floral transition pathways and hormonal signaling were regulated predominantly during the vegetative phase. Thus, SVP regulates many developmental pathways, some of which are common to both of its developmental roles whereas others are specific to only one of them.

【 授权许可】

   
2013 Gregis et al.; licensee BioMed Central Ltd.

【 图 表 】

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