Digital gene expression analysis during floral transition in pak choi (Brassica rapa subsp. chinensis)

Biotechnology & Biotechnological Equipment

Xueting Wang¹  Hongxia Song¹  Xiaoyong Xu¹  Mengxia Sun¹  Meilan Li¹  Guoming Xing¹  Leiping Hou¹  Meiying Gao¹  Zhujun Zhu² 

[1] Shanxi Agricultural University;Zhejiang A&F University;

关键词: Brassica rapa subsp. chinensis;    floral transition;    digital gene expression;   

DOI  :  10.1080/13102818.2017.1307141

来源: DOAJ

【 摘 要 】

Digital gene expression profiling technology was used to examine the changes in gene expression during floral transition in pak choi. A total of 1486 differentially expressed genes (DEGs) during floral transition were identified, of which 505 were upregulated and 981 were downregulated. Gene ontology enrichment analysis showed that the proteins encoded by DEGs were mainly located in eight cell regions including the apoplast, plant-type cell wall, chloroplast, etc. They had eight kinds of molecular functions such as transcription factor and oxidoreductase, and involved in 72 biological processes containing jasmonic acid and salicylic acid metabolism, glucose metabolism, the MAPK cascade, etc. Further pathway enrichment analysis showed that four metabolic pathways were significantly enriched by DEGs. DEGs that exhibited at least a 10-fold difference were analyzed in detail. Results showed that flavonoid metabolism and phenylpropanoid biosynthesis (or pathways) were significantly enriched and the corresponding gene number was the highest. Moreover, 15 genes among the highly expressed genes were involved in reproductive development, of which five homologues in Arabidopsis thaliana were directly related to floral transition, which were predicted to have a similar function in pak choi.

【 授权许可】

Unknown