Insights Into the Molecular Mechanisms of Late Flowering in Prunus sibirica by Whole-Genome and Transcriptome Analyses

Frontiers in Plant Science

Plant Science

Wenquan Bao¹  Mengzhen Huang²  Gaopu Zhu²  Ningning Gou²  Chen Chen²  Wanyu Xu²  Haikun Bai²  Huimin Liu²  Yixiao Chen²  Lin Wang²  Han Zhao²  Ta-na Wuyun² 

[1] College of Forestry, Inner Mongolia Agricultural University, Hohhot, China;State Key Laboratory of Tree Genetics and Breeding, Non-timber Forest Research and Development Center, Chinese Academy of Forestry, Zhengzhou, China;Kernel-Apricot Engineering and Technology Research Center of State Forestry and Grassland Administration, Zhengzhou, China;Key Laboratory of Non-timber Forest Germplasm Enhancement and Utilization of National Forestry and Grassland Administration, Zhengzhou, China;

关键词: TPP;    Tre6P;    TPS;    floral transition;    GWAS;    ceRNA;   

DOI  :  10.3389/fpls.2021.802827

 received in 2021-10-27, accepted in 2021-12-06,  发布年份 2022

来源: Frontiers

【 摘 要 】

Freezing during the flowering of Prunus sibirica is detrimental to fruit production. The late flowering (LF) type, which is delayed by 7–15 days compared with the normal flowering (NF) type, avoids damages at low temperature, but the molecular mechanism of LF remains unclear. Therefore, this study was conducted to comprehensively characterize floral bud differentiation. A histological analysis showed that initial floral bud differentiation was delayed in the LF type compared to the NF type. Genome-wide associated studies (GWAS) showed that a candidate gene (PaF106G0600023738.01) was significantly associated with LF type. It was identified as trehalose-6-phosphate phosphatase (PsTPPF), which is involved in trehalose-6-phosphate (Tre6P) signaling pathway and acts on floral transition. A whole-transcriptome RNA sequencing analysis was conducted, and a total of 6,110 differential expression (DE) mRNAs, 1,351 DE lncRNAs, and 148 DE miRNAs were identified. In addition, 24 DE mRNAs related with floral transition were predicted, and these involved the following: three interactions between DE lncRNAs and DE mRNAs of photoperiod pathway with two mRNAs (COP1, PaF106G0400018289.01 and CO3, MXLOC_025744) and three lncRNAs (CCLR, LTCONS_00031803, COCLR1, LTCONS_00046726, and COCLR2, LTCONS_00046731); one interaction between DE miRNAs and DE mRNAs with one mRNA, encoding trehalose-6-phosphate synthase (PsTPS1, PaF106G0100001132.01), and one miRNA (miRNA167h). Combined with the expression profiles and Tre6P levels, functions of PsTPPF and PsTPS1 in Tre6P regulation were considered to be associated with flowering time. A new network of ceRNAs correlated with LF was constructed, and it consisted of one mRNA (PsTPS1), one lncRNA (TCLR, LTCONS_00034157), and one miRNA (miR167h). This study provided insight into the molecular regulatory mechanism of LF in Prunus sibirica.

【 授权许可】

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Copyright © 2022 Xu, Bao, Liu, Chen, Bai, Huang, Zhu, Zhao, Gou, Chen, Wang and Wuyun.