The shape of the radiation dose response for DNA double-strand break induction and repair

Genome Integrity

Kai Rothkamm¹  Simon Bouffler¹  Stephen Barnard¹ 

[1] Health Protection Agency Centre for Radiation, Chemical and Environmental Hazards, Chilton, Didcot, Oxon OX11 0RQ, UK

关键词: Low dose;    Dose response;    Gamma-H2AX;    Pulsed-field gel electrophoresis;    Ionising radiation;    DNA double-strand break;   

Others  :  814634
DOI  :  10.1186/2041-9414-4-1

 received in 2013-02-12, accepted in 2013-03-18,  发布年份 2013

【 摘 要 】

DNA double-strand breaks are among the most deleterious lesions induced by ionising radiation. A range of inter-connected cellular response mechanisms has evolved to enable their efficient repair and thus protect the cell from the harmful consequences of un- or mis-repaired breaks which may include early effects such as cell killing and associated acute toxicities and late effects such as cancer. A number of studies suggest that the induction and repair of double-strand breaks may not always occur linearly with ionising radiation dose. Here we have aimed to identify and discuss some of the biological and methodological factors that can potentially modify the shape of the dose response curve obtained for these endpoints using the most common assays for double-strand breaks, pulsed-field gel electrophoresis and microscopic scoring of radiation-induced foci.

【 授权许可】

   
2013 Barnard et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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