Liver myofibroblasts up-regulate monocyte CD163 expression via PGE2 during hepatitis B induced liver failure

Journal of Translational Medicine

Zhiliang Gao²  Yutian Chong²  Qiang Tai¹  Zhanlian Huang²  Chan Xie²  Yurong Gu²  Yubao Zheng²  Qiyi Zhao²  Jianyun Zhu²  Fenglan Wang³  Yinong Ye²  Min Zhang² 

[1] Department of Hepatic Surgury, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of China;Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-sen University, No 600 Tianhe Road, Guangzhou 510630, Guangdong Province, People’s Republic of China;Department of Infectious Diseases, The First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an, People’s Republic of China

关键词: Hepatitis B virus;    Liver myofibroblasts;    Liver failure;    CD163 expression;    Monocyte activation;   

Others  :  817757
DOI  :  10.1186/1479-5876-12-60

 received in 2013-11-21, accepted in 2014-02-27,  发布年份 2014

【 摘 要 】

Background

Although patients with liver failure exhibit a generalized inflammatory-imbalance status, substantial evidence indicates that this immunosuppressive or anti-inflammatory state may be deleterious. Increased expression of CD163 (known to be involved in several anti-inflammatory functions of the immune system) in patients with liver failure is significantly correlated with a fatal outcome. However, little is known of the regulatory mechanisms that influence the expression of CD163.

Methods

We assessed the expression of CD163 on monocytes from both circulating cells and the liver tissues of patients with hepatitis B induced liver failure using flow cytometry and isolated the myofibroblasts from diseased livers. The ability of human liver myofibroblasts to regulate CD163 expression on monocytes was studied in vitro.

Results

We showed that CD163⁺ monocytes were enriched primarily in diseased livers and that they were associated with liver myofibroblasts in the same area. Accordingly, liver myofibroblasts were significantly superior to normal skin fibroblasts in inducing the expression of CD163 on monocytes in vitro. Moreover, we found that liver myofibroblasts triggered the activation of monocytes by secreting PGE2. Inhibition of PGE2 production in liver myofibroblasts using NS-398 markedly reduced CD163 expression in vitro.

Conclusion

These results suggest that liver myofibroblasts play a direct role in regulating the expression of CD163 on monocytes in human liver tissues and thereby may regulate monocyte function during hepatitis B induced liver failure.

【 授权许可】

   
2014 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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