Liver myofibroblasts from hepatitis B related liver failure patients may regulate natural killer cell function via PGE2

Journal of Translational Medicine

Zhiliang Gao³  Shumei Lin²  Liang Peng³  Yubao Zheng³  Qiyi Zhao³  Qiang Tai¹  Yutian Chong³  Fenglan Wang⁴  Min Zhang³ 

[1] Department of Hepatic Surgury, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China;Department of Infectious Diseases, First Affiliated Hospital of Medical College of Xi?an Jiaotong University, No. 277 Yanta West Road, Xi?an 710061, Shanxi Province, China;Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China;Department of Infectious Diseases, the Eighth Hospital of Xi?an, Xi?an, China

关键词: Hepatitis B;    Liver failure;    Immune-mediated liver injury;    Natural killer cell;    Liver myofibroblasts;   

Others  :  1147354
DOI  :  10.1186/s12967-014-0308-9

 received in 2014-07-31, accepted in 2014-10-22,  发布年份 2014

【 摘 要 】

Background

Natural killer (NK) cells are abundant in the liver and constitute a major innate immune component that contributes to immune-mediated liver injury. However, few studies have investigated the phenotypes and functions of NK cells involved in hepatitis B related liver failure (LF), and the precise mechanism underlying NK cell regulation is not fully understood.

Methods

We detected the percentage and function of peripheral NK cells both in hepatitis B related LF patients and healthy volunteers by flow cytometry and isolated the liver myofibroblasts (LMFs) from hepatitis B related LF livers. To determine the possible effects of LMFs on NK cells, mixed cell cultures were established in vitro.

Results

We found a down-regulated percentage of peripheral NK cells in hepatitis B related LF patients, and their NK cells also displayed decreased activated natural cytotoxicity receptors (NCRs) and cytokine production. In a co-culture model, LMFs sharply attenuated IL-2-induced NK cell triggering receptors, cytotoxicity, and cytokine production. The inhibitory effect of LMFs on NK cells correlated with their ability to produce prostaglandin (PG) E2.

Conclusion

These data suggest that LMFs may protect against immune-mediated liver injury in hepatitis B related LF patients by inhibiting NK cell function via PGE2.

【 授权许可】

   
2014 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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