Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach

Journal of Biological Engineering

Charles D Miller¹  Ronald C Sims¹  Alex D Hatch¹  Elisabeth Linton¹  Asif Rahman¹ 

[1] Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan 84322-4105, UT, USA

关键词: Synthetic biology;    HlyA;    Translocation;    Phasin;    Secretion;    PHB;    PHA;    Polyhydroxyalkanoates;   

Others  :  805219
DOI  :  10.1186/1754-1611-7-24

 received in 2013-06-28, accepted in 2013-09-12,  发布年份 2013

【 摘 要 】

Background

Polyhydroxyalkanoates (PHAs) are a group of biodegradable plastics that are produced by a wide variety of microorganisms, mainly as a storage intermediate for energy and carbon. Polyhydroxybutyrate (PHB) is a short-chain-length PHA with interesting chemical and physical properties. Large scale production of PHB is not wide-spread mainly due to the downstream processing of bacterial cultures to extract the PHB. Secretion of PHB from Escherichia coli could reduce downstream processing costs. PHB are non-proteinaceous polymers, hence cannot be directly targeted for secretion. Phasin, PhaP1, is a low molecular weight protein that binds to PHB, reducing PHB granule size. In this study PHB is indirectly secreted with PhaP1 from E. coli via type I secretion using HlyA signal peptides.

Results

This study demonstrated the successful secretion of phasin and phasin bound PHB outside of the cell and into the culture medium. The secretion of PHB was initiated between 24 and 48 h after induction. After 48 h of culturing, 36% of the total PHB produced in the secreting strain was collected in the secreted fraction and 64% remained in the internal fraction. To further support the findings of this study, the PHB secretion phenomenon was observed using SEM.

Conclusions

From this study, the ability to use type I secretion to: 1) secrete phasin and 2) successfully secrete PHB has been shown.

【 授权许可】

   
2013 Rahman et al.; licensee BioMed Central Ltd.

【 图 表 】

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