PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli

Emerging Infectious Diseases

关键词: Stx1;    Stx2;    eae;    ehlx;    HlyA;    ToxB;   

DOI  :  10.3201/eid1308.060428

来源: DOAJ

【 摘 要 】

We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.

【 授权许可】

Unknown