Protective effects of Sonchus asper against KBrO3 induced lipid peroxidation in rats

Lipids in Health and Disease

Sumaira Sahreen²  Muhammad Rashid Khan¹  Rahmat Ali Khan³ 

[1] Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan;Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan;Department of Biotechnology, Faculty of Sciences, University of Science and Technology Bannu, Bannu, Pakistan

关键词: TSH;    DNA fragmentation;    FSH;    Antioxidant;    Sonchus asper;    Potassium bromate;   

Others  :  1160131
DOI  :  10.1186/1476-511X-11-164

 received in 2012-08-21, accepted in 2012-11-20,  发布年份 2012

【 摘 要 】

Background

Sonchus asper is traditionally used in Pakistan for the treatment of reproductive dysfunction and oxidative stress. The present investigation was aimed to evaluate chloroform extract of Sonchus asper (SACE) against potassium bromate-induced reproductive stress in male rats.

Methods

20 mg/kg body weight (b.w.) potassium bromate (KBrO₃) was induced in 36 rats for four weeks and checked the protective efficacy of SACE at various hormonal imbalances, alteration of antioxidant enzymes, and DNA fragmentation levels. High performance chromatography (HPLC) was used for determination of bioactive constituents responsible.

Results

The level of hormonal secretion was significantly altered by potassium bromate. DNA fragmentation%, activity of antioxidant enzymes; catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and phase II metabolizing enzymes viz; glutathione reductase (GSR), glutathione peroxidase (GSHpx), glutathione-S-tansase (GST) and reduced glutathione (GSH) was decreased while hydrogen per oxide contents and thiobarbituric acid reactive substances (TBARS) were increased with KBrO₃ treatment. Treatment with SACE effectively ameliorated the alterations in the biochemical markers; hormonal and molecular levels while HPLC characterization revealed the presence of catechin, kaempferol, rutin and quercetin.

Conclusion

Protective effects of Sonchus asper vs. KBrO₃ induced lipid peroxidation might be due to bioactive compound present in SACE.

【 授权许可】

   
2012 Khan et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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