Protective effects of Sonchus asper against KBrO3 induced lipid peroxidation in rats

Lipids in Health and Disease

Research

Sumaira Sahreen¹  Muhammad Rashid Khan²  Rahmat Ali Khan³ 

[1] Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan;Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan;Department of Biotechnology, Faculty of Sciences, University of Science and Technology Bannu, Bannu, Pakistan;

关键词: Potassium bromate;    Sonchus asper;    Antioxidant;    FSH;    DNA fragmentation;    TSH;   

DOI  :  10.1186/1476-511X-11-164

 received in 2012-08-21, accepted in 2012-11-20,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundSonchus asper is traditionally used in Pakistan for the treatment of reproductive dysfunction and oxidative stress. The present investigation was aimed to evaluate chloroform extract of Sonchus asper (SACE) against potassium bromate-induced reproductive stress in male rats.Methods20 mg/kg body weight (b.w.) potassium bromate (KBrO3) was induced in 36 rats for four weeks and checked the protective efficacy of SACE at various hormonal imbalances, alteration of antioxidant enzymes, and DNA fragmentation levels. High performance chromatography (HPLC) was used for determination of bioactive constituents responsible.ResultsThe level of hormonal secretion was significantly altered by potassium bromate. DNA fragmentation%, activity of antioxidant enzymes; catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and phase II metabolizing enzymes viz; glutathione reductase (GSR), glutathione peroxidase (GSHpx), glutathione-S-tansase (GST) and reduced glutathione (GSH) was decreased while hydrogen per oxide contents and thiobarbituric acid reactive substances (TBARS) were increased with KBrO3 treatment. Treatment with SACE effectively ameliorated the alterations in the biochemical markers; hormonal and molecular levels while HPLC characterization revealed the presence of catechin, kaempferol, rutin and quercetin.ConclusionProtective effects of Sonchus asper vs. KBrO3 induced lipid peroxidation might be due to bioactive compound present in SACE.

【 授权许可】

CC BY   
© Khan et al.; licensee BioMed Central Ltd. 2012