Variation in time and magnitude of immune response and viremia in experimental challenges with Porcine circovirus 2b

BMC Veterinary Research

Daniel C Ciobanu³  Stephen D Kachman¹  Graham S Plastow²  Thomas E Burkey³  Judith A Galeota⁵  Emily H Davis³  Sarah Y Barnes³  Justin W Bundy³  Autumn M McKnite³  Timothy W Moural⁴  Erin E Jobman³  Taylor B Engle³ 

[1] Department of Statistics, University of Nebraska, Lincoln, USA;Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada;Animal Science Department, University of Nebraska, Lincoln 68583-0908, NE, USA;Bluegrass Community & Technical College, Lexington, USA;School of Veterinary Medicine and Biological Sciences, University of Nebraska, Lincoln, USA

关键词: Swine;    PCV2b;    Disease susceptibility;   

Others  :  1091981
DOI  :  10.1186/s12917-014-0286-4

 received in 2014-08-06, accepted in 2014-11-19,  发布年份 2014

【 摘 要 】

Background

Porcine circovirus 2 is the primary agent responsible for inducing a group of associated diseases known as Porcine Circovirus Associated Diseases (PCVAD), which can have detrimental effects on production efficiency as well as causing significant mortality. The objective of this study was to evaluate variation in viral replication, immune response and growth across pigs (n = 974) from different crossbred lines. The approach used in this study was experimental infection with a PCV2b strain of pigs at an average of 43 days of age.

Results

The sequence of the PCV2b isolate used in the challenge was similar with a cluster of PCV2b isolates known to induce PCVAD and increased mortality rates. The swine leukocyte antigen class II (SLAII) profile of the population was diverse, with nine DQB1 haplotypes being present. Individual viremia and antibody profiles during challenge demonstrate variation in magnitude and time of viral surge and immune response. The correlations between PCV2 specific antibodies and average daily gain (ADG) were relatively low and varied between - 0.14 to 0.08 for IgM and −0.02 and 0.11 for IgG. In contrast, PCV2 viremia was an important driver of ADG decline following infection; a moderate negative correlation was observed between viral load and overall ADG (r = − 0.35, P < 0.001). The pigs with the lowest 10% level of viral load maintained a steady increase in weekly ADG (P < 0.0001) compared to the pigs that had the 10% greatest viral load (P < 0.55). In addition, the highly viremic group expressed higher IgM and IgG starting with d 14 and d 21 respectively, and higher tumor necrosis factor – alpha (TNF-α) at d 21 (P < 0.005), compared to low viremic group.

Conclusions

Molecular sources of the observed differences in viremia and immune response could provide a better understanding of the host factors that influence the development of PCVAD and lead to improved knowledge of swine immunity.

【 授权许可】

   
2014 Engle et al.; licensee BioMed Central Ltd.

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【 参考文献 】

• [1]Madec F, Rose N, Grasland B, Cariolet R, Jestin A: Post-weaning multisystemic wasting syndrome and other PCV2-related problems in pigs: a 12-year experience. Transbound Emerg Dis 2008, 55:273-283.
• [2]Bates JS, Moreno R, Doster AR, Johnson RK: Selection for immune response to porcine circovirus (PCV2) to decrease incidence of porcine circovirus associated disease. Nebraska Swine Report 2009, EC219:33-38.
• [3]Cino-Ozuna AG, Henry S, Hesse R, Nietfeld JC, Bai J, Scott HM, Rowland RR: Characterization of a new disease syndrome associated with porcine circovirus type 2 in previously vaccinated herds. J Clin Microbiol 2011, 49(5):2012-2016.
• [4]Zhou JY, Chen QX, Ye JX, Shen HG, Chen TF, Shang SB: Serological investigations and genomic characterization of PCV2 isolates from different geographic regions of Zhejiang province in China. Vet Res Commun 2006, 30:205-220.
• [5]Opriessnig T, Fenaux M, Thomas P, Hoogland MJ, Rothschild MF, Meng XJ, Halbur PG: Evidence of breed-dependent differences in susceptibility to porcine circovirus type-2-associated disease and lesions. Vet Pathol 2006, 43(3):281-293.
• [6]Opriessnig T, Patterson AR, Madson DM, Pal N, Rothschild M, Kuhar D, Lunney JK, Juhan NM, Meng XJ, Halbur PG: Difference in severity of porcine circovirus type two-induced pathological lesions between Landrace and Pietrain pigs. J Anim Sci 2009, 87(5):1582-1590.
• [7]McKnite AM, Bundy JW, Moural TW, Tart JK, Johnson TP, Jobman EE, Barnes SY, Qiu JK, Peterson DA, Harris SP, Rothschild MF, Galeota JA, Johnson RK, Kachman SD, Ciobanu DC: Genomic analysis of the differential response to experimental infection with porcine circovirus 2b. Anim Genet 2014, 45(2):205-214.
• [8]Lunney JK, Ho CS, Wysocki M, Smith DM: Molecular genetics of the swine major histocompatibility complex, the SLA complex. Dev Comp Immunol 2009, 33(3):362-374.
• [9]Serao NV, Matika O, Kemp RA, Harding JC, Bishop SC, Plastow GS, Dekkers JC: Genetic analysis of reproductive traits and antibody response in a PRRS outbreak herd. J Anim Sci 2014, 92(7):2905-2921.
• [10]Ho CS, Lunney JK, Lee JH, Franzo-Romain MH, Martens GW, Rowland RR, Smith DM: Molecular characterization of swine leucocyte antigen class II genes in outbred pig populations. Anim Genet 2010, 41(4):428-432.
• [11]Opriessnig T, Madson DM, Prickett JR, Kuhar D, Lunney JK, Elsener J, Halbur PG: Effect of porcine circovirus type 2 (PCV2) vaccination on porcine reproductive and respiratory syndrome virus (PRRSV) and PCV2 coinfection. Vet Microbiol 2008, 131(1–2):103-114.
• [12]Carman S, Cai HY, DeLay J, Youssef SA, McEwen BJ, Gagnon CA, Tremblay D, Hazlett M, Lusis P, Fairles J, Alexander HS, van Dreumel T: The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease–2004–2006. Can J Vet Res 2008, 72(3):259-268.
• [13]Gagnon CA, Tremblay D, Tijssen P, Venne MH, Houde A, Elahi SM: The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada. Can Vet J 2007, 48(8):811-819.
• [14]Vincent IE, Balmelli C, Meehan B, Allan G, Summerfield A, McCullough KC: Silencing of natural interferon producing cell activation by porcine circovirus type 2 DNA. Immunology 2007, 120(1):47-56.
• [15]Vincent IE, Carrasco CP, Guzylack-Piriou L, Herrmann B, McNeilly F, Allan GM, Summerfield A, McCullough KC: Subset-dependent modulation of dendritic cell activity by circovirus type 2. Immunology 2005, 115(3):388-398.
• [16]Allan GM, Ellis JA: Porcine circoviruses: a review. J Vet Diagn Invest 2000, 12:3-14.
• [17]McKeown NE, Opriessnig T, Thomas P, Guenette DK, Elvinger F, Fenaux M, Halbur PG, Meng XJ: Effects of porcine circovirus type 2 (PCV2) maternal antibodies on experimental infection of piglets with PCV2. Clin Diagn Lab Immunol 2005, 12(11):1347-1351.
• [18]Mastromano GA, Li YS, Tran H, Bundy JW, Ciobanu DC, Miller PS, Burkey TE: Diet complexity affects post-weaning performance and immune response to Porcine Circovirus 2 (PCV2) infection in nursery pigs. Journal Animal Science 2014, 92(E-Suppl. 2):162.
• [19]Pensaert MB, Sanchez RE Jr, Ladekjaer-Mikkelsen AS, Allan GM, Nauwynck HJ: Viremia and effect of fetal infection with porcine viruses with special reference to porcine circovirus 2 infection. Vet Microbiol 2004, 98(2):175-183.
• [20]Opriessnig T, Thacker EL, Yu S, Fenaux M, Meng XJ, Halbur PG: Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2. Vet Pathol 2004, 41(6):624-640.
• [21]Meerts P, Van Gucht S, Cox E, Vandebosch A, Nauwynck HJ: Correlation between type of adaptive immune response against porcine circovirus type 2 and level of virus replication. Viral Immunol 2005, 18(2):333-341.
• [22]Kreikemeier CA, Engle TB, Lucot KL, Kachman SD, Burkey TE, Ciobanu DC: Genome-wide Analysis of TNF-alpha Response in Pigs Challenged with Porcine Circovirus 2b.Anim Genet. in press.
• [23]Trevejo JM, Marino MW, Philpott N, Josien R, Richards EC, Elkon KB, Falck-Pedersen E: TNF-alpha -dependent maturation of local dendritic cells is critical for activating the adaptive immune response to virus infection. Proc Natl Acad Sci U S A 2001, 98(21):12162-12167.
• [24]Fort M, Olvera A, Sibila M, Segales J, Mateu E: Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Vet Microbiol 2007, 125(3–4):244-255.
• [25]Meerts P, Misinzo G, Lefebvre D, Nielsen J, Botner A, Kristensen CS, Nauwynck HJ: Correlation between the presence of neutralizing antibodies against porcine circovirus 2 (PCV2) and protection against replication of the virus and development of PCV2-associated disease. BMC Vet Res 2006, 2:6. BioMed Central Full Text
• [26]Boddicker N, Waide EH, Rowland RR, Lunney JK, Garrick DJ, Reecy JM, Dekkers JC: Evidence for a major QTL associated with host response to porcine reproductive and respiratory syndrome virus challenge. J Anim Sci 2012, 90(6):1733-1746.
• [27]Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: Clustal W and Clustal X version 2.0. Bioinformatics 2007, 23(21):2947-2948.