Journal of Translational Medicine | |
Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis | |
Jing-Ping Yun1  Yan Li1  Mu-Yan Cai1  Guo-Bing Xie1  Xuan Zhou1  Li-Li Liu1  Chun Yi1  Chris Zhi-Yi Zhang1  Lin-Zi Li1  Yan Huang1  | |
[1] Department of Pathology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, China, Sun Yat-Sen University Cancer Center, No 651, East Dongfeng Road, Guangzhou, 510060, China | |
关键词: Metastasis; Recurrence; Prognosis; Hepatocellular carcinoma; AZGP1; | |
Others : 1205975 DOI : 10.1186/1479-5876-10-106 |
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received in 2012-03-15, accepted in 2012-05-09, 发布年份 2012 | |
【 摘 要 】
Background
Zinc-alpha2-glycoprotein (AZGP1, ZAG) was recently demonstrated to be an important factor in tumor carcinogenesis. However, AZGP1 expression in hepatocellular carcinoma (HCC) and its significance remain largely unknown.
Methods
Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to determine mRNA level of AZGP1 in 20 paired fresh HCC tissues. Clinical and pathological data of 246 HCC patients were collected. Tissue-microarray-based immunohistochemistry (IHC) was performed to examine AZGP1 expression in HCC samples. Relationship between AZGP1 expression and clinicopathological features was analyzed by Chi-square test, Kaplan-Meier analysis and Cox proportional hazards regression model.
Results
AZGP1 expression was significantly lower in 80.0% (16/20) of tumorous tissues than that in the corresponding adjacent nontumorous liver tissues (P < 0.001). Consistently, IHC data revealed that decreased expression of AZGP1 was present in 80.1% (197/246) of HCC patient tissues (P < 0.001). Furthermore, AZGP1 expression in HCC significantly associated with several clinicopathological parameters, including serum AFP level (P = 0.013), liver cirrhosis (P = 0.002) and tumor differentiation (P = 0.025). Moreover, HCC patients with high AZGP1 expression survived longer, with better overall survival (P = 0.006) and disease-free survival (P = 0.025). In addition, low AZGP1 expression associated with worse relapse-free survival (P = 0.046) and distant metastatic progression-free survival (P = 0.036).
Conclusion
AZGP1 was downregulated in HCC and could be served as a promising prognostic marker for HCC patients.
【 授权许可】
2012 Huang et al.; licensee BioMed Central Ltd.
【 预 览 】
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