Piwi-interacting RNAs as novel prognostic markers in clear cell renal cell carcinomas

Journal of Experimental & Clinical Cancer Research

Klaus Jung⁶  Annika Fendler²  Ergin Kilic⁴  Philipp Schwabe¹  Elena Trujillo-Arribas⁵  Zofia Wotschofsky⁶  Monika Jung³  Bernhard Ralla³  Jonas Busch³ 

[1] Center for Musculoskeletal Surgery, University Hospital Charité, Berlin, Germany;Department of Signal Transduction, Invasion and Metastasis of Epithelial Cells, Max Delbrück Center of Molecular Medicine, Berlin, Germany;Department of Urology, Research Division, University Hospital Charité, Schumannstr. 20/12, Berlin, 10117, Germany;Institute of Pathology, University Hospital Charité, Berlin, Germany;Laboratorio clinico, Hospital Universitario Virgen Del Rocio, Sevilla, Spain;Berlin Institute for Urologic Research, Berlin, Germany

关键词: Overall survival;    Recurrence;    Metastasis;    Prognosis;    piRNA;    Clear cell renal cell carcinoma;   

Others  :  1220677
DOI  :  10.1186/s13046-015-0180-3

 received in 2015-03-08, accepted in 2015-06-05,  发布年份 2015

【 摘 要 】

Background

Piwi-interacting RNAs (piRNAs) are small RNAs of 27–30 nucleotides mapping to transposons or clustering in repeat genomic regions. Preliminary studies suggest an important role in cancerogenesis. This study is the first one investigating their prognostic impact in clear cell renal cell cancer (ccRCC) patients.

Methods

Three piRNAs (piR-30924, piR-57125, and piR-38756) selected on the basis of initial piRNA microarray analyses were determined using RT-qPCR in non-metastatic (n = 76) and metastatic (n = 30) ccRCC tissue at the time of nephrectomy in comparison to normal renal tissue (n = 77) and tissue from distant ccRCC metastases (n = 13). Primary clinical end points were recurrence-free and overall survival.

Results

piR-57125 showed lower expression in metastatic than in non-metastatic tumors, whereas the expression of piR-30924 and piR-38756 increased in metastatic tumors. The higher expression of piR-30924 and piR-38756 as well as the lower expression of piR-57125 in metastatic primary tumors were significantly associated with tumor recurrence and overall survival. Multivariate Cox regression analyses revealed both piR-30924 and piR-57125 as independent prognostic predictors. This impact was even more pronounced in non-metastatic patients.

Conclusions

This study demonstrates that the expression levels of these piRNAs in primary non-metastatic and metastatic ccRCC tissue can serve as potential prognostic biomarkers in combination with clinicopathological factors.

【 授权许可】

   
2015 Busch et al.

【 图 表 】

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