Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells

Cancer Cell International

Leila Chekir Ghedira²  Kamel Ghedira²  Ines Skandrani¹  Jihed Boubaker¹  Wissem Bhouri¹ 

[1] Unité de pharmacognosie 99/UR/07, Faculté de pharmacie Monastir, Rue Avicenne, Monastir, 5000 Tunisia;Faculty of Dental Medicine, Rue Avicenne, Monastir, 5019, Tunisia

关键词: PARP;    Digallic acid;    DNA fragmentation;    Caspase activity;   

Others  :  794934
DOI  :  10.1186/1475-2867-12-26

 received in 2011-11-15, accepted in 2012-05-29,  发布年份 2012

【 摘 要 】

Background

The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.

Methods

We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.

Results

The inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.

Conclusion

In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.

【 授权许可】

   
2012 Bhouri et al.; licensee BioMed Central Ltd.

【 图 表 】

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