Antioxidant and cytotoxic activities of three species of tropical seaweeds

BMC Complementary and Alternative Medicine

Wai Sum Yap³  Hwee Ming Cheng⁵  Jayakumar Rajarajeswaran⁴  Kong Soo Khoo¹  M S Kanthimathi²  Yin Yin Chia⁴ 

[1] Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman (Kampar campus), Jalan Universiti, Bandar Barat, Kampar, 31900, Perak, Malaysia;University of Malaya Centre for Proteomics Research (UMCPR), University of Malaya, Kuala Lumpur, Malaysia;Department of Biotechnology, Faculty of Applied Sciences, UCSI University, 56000 UCSI Heights, Kuala Lumpur, Malaysia;Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia;Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia

关键词: DNA fragmentation;    Caspase activity;    LC-MS;    Antioxidant enzyme activity;    Antioxidant activity;    MTT assay;    Cytotoxic activity;    Turbinaria ornata;    Caulerpa racemosa;    Padina tetrastromatica;   

Others  :  1233547
DOI  :  10.1186/s12906-015-0867-1

 received in 2015-02-25, accepted in 2015-09-18,  发布年份 2015

【 摘 要 】

Background

Three species of seaweeds (Padina tetrastromatica, Caulerpa racemosa and Turbinaria ornata) are widely consumed by Asians as nutraceutical food due to their antioxidant properties. Studies have shown that these seaweeds exhibit bioactivities which include antimicrobial, antiviral, anti-hypertensive and anticoagulant activities. However, investigations into the mechanisms of action pertaining to the cytotoxic activity of the seaweeds are limited. The aim of this study was to determine the antioxidant and cytotoxic activities of whole extracts of P. tetrastromatica, C. racemosa and T. ornata, including the cellular events leading to the apoptotic cell death of the extract treated-MCF-7 cells. Bioassay guided fractionation was carried out and the compounds identified.

Methods

Powdered samples were sequentially extracted for 24 h. Their antioxidant activities were assessed by the DPPH radical, superoxide, nitric oxide and hydroxyl radical scavenging assays. The cytotoxic activity of the extract-treated MCF-7cells was assessed using the MTT assay. The most potent fraction was subjected to bioassay guided fractionation with column chromatography. All the fractions were tested for cytotoxic activity, caspase activity and effect on DNA fragmentation.

Results

All three seaweeds showed potent radical scavenging activities in the various assays. The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner. The partially purified fractions exhibited higher cytotoxic activity, as assessed by the MTT assay, than the whole extracts in the breast adenocarcinoma cell line, MCF-7. LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine.

Conclusion

Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits.

【 授权许可】

   
2015 Chia et al.

【 图 表 】

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