Diagnostic Pathology | |
A critical role of mir-199a in the cell biological behaviors of colorectal cancer | |
Xuebao Zheng1  Ying Deng1  Cancan Guo1  Yuzhen Zhu1  Qiong Wu1  Liping Pang1  Hua Ye1  | |
[1] Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China | |
关键词: Vascular endothelial growth factor; Hypoxia-inducible factor 1α; In vitro; Cell lines; Colorectal cancer; MiR-199a; | |
Others : 1217731 DOI : 10.1186/s13000-015-0260-x |
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received in 2014-11-25, accepted in 2015-04-06, 发布年份 2015 | |
【 摘 要 】
Background
Colorectal cancer (CRC) is one of the most common cancer and the leading causes of cancer mortality worldwide. The critical role of hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) are important in the cancer development.
Methods
The purpose of this study was to investigate the association of miR-199a expression in CRC and non-tumor tissues as well as assessed the effect of miR-199a on biological behaviors including cell proliferation, apoptosis, migration and invasion of CRC cells. The expression of miR-199a was distinctly decreased in colorectal cancer tissues compared with non-neoplastic colorectal tissues.
Results
In this study, we found that miR-199a down-regulation was associated with the CRC and metastasis incidence. Advanced study showed that miR-199a up-regulation would lead to decreased CRC proliferation, migration and invasion. However, no significant association of miR-199a treatment and apoptosis rate and cell-cycle were detected in this study. The detection for the mechanisms of miR-199a on the development of CRC showed that the anticarcinogenic effect of miR-199a might be produced through HIF-1α/VEGF pathway.
Conclusion
It was found that miR-199a would reduce the proliferation, migration and invasion. However, overexpression of miR-199a on the apoptosis rate and cell cycles showed no significant results. The potential functionary mechanism of miR-199a might through HIF-1α/VEGF pathway.
Virtual slides
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/9806714131513041.
【 授权许可】
2015 Ye et al.
【 预 览 】
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