Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results

Journal of Neuroinflammation

Tomoki Hashimoto¹  Pascal Jabbour³  Nohra Chalouhi³  David Hasan² 

[1] Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco, CA, USA;Department of Neurosurgery, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, JCP 1616, Iowa City, IA, 52242, USA;Department of Neurosurgery, Thomas Jefferson University and Jefferson Hospital for Neuroscience, Philadelphia, PA, USA

关键词: Mast cells;    M2;    M1;    Macrophages;    Inflammation;    Aneurysm;   

Others  :  1160238
DOI  :  10.1186/1742-2094-9-222

 received in 2012-07-17, accepted in 2012-09-12,  发布年份 2012

【 摘 要 】

Background

M1 and M2 cells are two major subsets of human macrophages that exert opposite effects on the inflammatory response. This study aims to investigate the role of macrophage M1/M2 imbalance and mast cells in the progression of human cerebral aneurysms to rupture.

Methods

Ten patients with cerebral aneurysms (five ruptured and five unruptured) underwent microsurgical clipping. During the procedure, a segment of the aneurysm dome was resected and immunostained with monoclonal antibodies for M1 cells (anti-HLA DR), M2 cells (anti-CD 163), and mast cells (anti-tryptase clone AA). A segment of the superficial temporal artery (STA) was also removed and immunostained with monoclonal antibodies for M1, M2, and mast cells.

Results

All ten aneurysm tissues stained positive for M1, M2, and mast cells. M1 and M2 cells were present in equal proportions in unruptured aneurysms. This contrasted with a marked predominance of M1 over M2 cells in ruptured aneurysms (p = 0.045). Mast cells were also prominently upregulated in ruptured aneurysms (p = 0.001). Few M1 and M2 cells were present in STA samples.

Conclusions

M1/M2 macrophages and mast cells are found in human cerebral aneurysms; however, M1 and mast cell expression seems to markedly increase in ruptured aneurysms. These findings suggest that macrophage M1/M2 imbalance and upregulation of mast cells may have a role in the progression of cerebral aneurysms to rupture.

【 授权许可】

   
2012 Hasan et al.; licensee BioMed Central Ltd.

【 图 表 】

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