CHARACTERIZATION OF INFLUENZA A VIRUS M2 CYTOPLASMICTAIL AMINO ACID RESIDUE POSITION 83 AND 86 MUTATIONS

influenza;M2;cytoplasmic tail;mutations;characterization;not listed

Ye, YangPekosz, Andrew S. ;

Johns Hopkins University

关键词: influenza;    M2;    cytoplasmic tail;    mutations;    characterization;    not listed;   

Others  :  https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/39476/YE-THESIS-2016.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: JOHNS HOPKINS DSpace Repository

【 摘 要 】

Influenza is a world-wide infectious disease caused by influenza virus and results in much death annually. One of the key methods contributing to the prevention of this disease is influenza vaccines, which include live attenuated influenza vaccine(LAIV). Although the sequence of LAIV strain has been revealed more than 15 years, we still do not fully understand which part of LAIV mutated genes mainly contribute to the phenotype of attenuation. Based on prior researches, we tested the hypothesis that the amino acid(aa) residue position 83 and 86 of influenza A virus M2 cytoplasmic tail domain are responsible for virus replication. To study that, we generated panels of viruses with single amino acid mutations at target position based on A/Udorn/72 influenza A virus backbone. With verifying their sequences, plaques in MDCK cells and growth curves in both MDCK cells and primary human nasal epithelial cells are produced for identifying characteristics of different mutations. We conclude that specific mutations at position 83 and 86 of M2 protein cytoplasmic tail domain can change the kinetics and amount of progeny virus produced in A/Udorn/72 influenza A virus replication.