Dual polarization of human alveolar macrophages progressively increases with smoking and COPD severity

Respiratory Research

Research

Marco Schiavon¹  Chiara Rigobello¹  Mariaenrica Tinè¹  Marina Saetta¹  Graziella Turato¹  Francesca Lunardi¹  Fiorella Calabrese¹  Davide Biondini¹  Elisabetta Balestro¹  Federico Rea¹  Simonetta Baraldo¹  Erica Bazzan¹  Manuel G. Cosio²  Paolo Simioni³  Claudia M. Radu³ 

[1] Department of Cardiac, Thoracic, and Vascular Sciences, University of Padova, Padova, Italy;Department of Cardiac, Thoracic, and Vascular Sciences, University of Padova, Padova, Italy;Respiratory Division, Meakins-Christie Laboratories, McGill University, Montreal, Canada;Department of Medicine, University of Padova, Padova, Italy;

关键词: COPD;    Cigarette smoking;    Macrophages;    M1;    M2;   

DOI  :  10.1186/s12931-017-0522-0

 received in 2016-12-13, accepted in 2017-02-14,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIt is known that tissue macrophages derive not only from blood monocytes but also from yolk sac or fetal liver, and the tissue of residence guides their function. When isolated, they lose tissue specific signatures, hence studies of human macrophages should be ideally done directly in the tissue. The aim of this study was to investigate directly in human lung tissue the polarization of alveolar macrophage (AM), classic (M1) or alternative (M2), in health and disease, using COPD as a model.MethodsSurgical lungs from 53 subjects were studied: 36 smokers whose FEV1 varied from normal to severe COPD, 11 non-smokers and 6 normal donors. iNOS and CD206 immunohistochemistry was used to quantify the percentage of AM polarized as M1 or M2 in lung sections.Results and DiscussionThe percentage of M1 and M2 increased progressively with smoking and COPD severity, from 26% to 84% for M1 and from 7% to 78% for M2. In donors 74% of AM were negative for M1 and 93% for M2. Confocal microscopy showed co-localization of M1 and M2 in the same AM in severe COPD.ConclusionIn normal lungs alveolar macrophages were mostly non-polarized. With smoking and COPD severity, M1 and M2 polarization increased significantly and so did the co-expression of M1 and M2 in the same alveolar macrophage.

【 授权许可】

CC BY   
© The Author(s). 2017