Characterization of adipose tissue macrophages and adipose-derived stem cells in critical wounds

PeerJ

article

Bong-Sung Kim¹  Pathricia V. Tilstam²  Katrin Springenberg-Jung¹  Arne Hendrick Boecker¹  Corinna Schmitz³  Daniel Heinrichs³  Soo Seok Hwang⁴  Jan Philipp Stromps¹  Bergita Ganse⁵  Ruedger Kopp⁶  Matthias Knobe⁵  Juergen Bernhagen⁷  Norbert Pallua¹  Richard Bucala² 

[1] Plastic and Reconstructive Surgery, Hand Surgery—Burn Center;Department of Medicine, Yale University;Institute of Biochemistry and Molecular Cell Biology;Department of Immunology, Yale University;Department of Orthopedic Trauma Surgery;Department of Intensive Care Medicine;Department of Vascular Biology, Institute for Stroke and Dementia Research, Ludwig-Maximilians-Universität München;Munich Cluster for Systems Neurology ,(SyNergy), Ludwig-Maximilians-Universität München

关键词: Wound repair;    Adipose tissue;    Inflammation;    Macrophages;    Adipose-derived stem cells;    Polarization;    M2;    M1;   

DOI  :  10.7717/peerj.2824

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundSubcutaneous adipose tissue is a rich source of adipose tissue macrophages and adipose-derived stem cells which both play a key role in wound repair. While macrophages can be divided into the classically-activated M1 and the alternatively-activated M2 phenotype, ASCs are characterized by the expression of specific stem cell markers.MethodsIn the present study, we have investigated the expression of common macrophage polarization and stem cell markers in acutely inflamed adipose tissue. Subcutaneous adipose tissue adjacent to acutely inflamed wounds of 20 patients and 20 healthy subjects were harvested and underwent qPCR and flow cytometry analysis.ResultsExpression levels of the M1-specific markers CD80, iNOS, and IL-1b were significantly elevated in inflammatory adipose tissue when compared to healthy adipose tissue, whereas the M2-specific markers CD163 and TGF-β were decreased. By flow cytometry, a significant shift of adipose tissue macrophage populations towards the M1 phenotype was confirmed. Furthermore, a decrease in the mesenchymal stem cell markers CD29, CD34, and CD105 was observed whereas CD73 and CD90 remained unchanged.DiscussionThis is the first report describing the predominance of M1 adipose tissue macrophages and the reduction of stem cell marker expression in acutely inflamed, non-healing wounds.

【 授权许可】

CC BY