Characterisation and comparison of adipose tissue macrophages from human subcutaneous, visceral and perivascular adipose tissue

Journal of Translational Medicine

Research

Marek Petras¹  Alena Sekerkova²  Rudolf Poledne³  Ivana Kralova Lesna³  Anna Kralova³  Sona Cejkova³  Libor Janousek⁴  Filip Thieme⁴  Jiri Fronek⁴ 

[1] 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic;Department of Clinical and Transplant Immunology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic;Laboratory for Atherosclerosis Research, Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic;Transplant Surgery Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic;

关键词: Adipose tissue;    Macrophages;    Inflammation;    Menopause;   

DOI  :  10.1186/s12967-016-0962-1

 received in 2016-04-26, accepted in 2016-06-27,  发布年份 2016

来源: Springer

【 摘 要 】

Background and aimsMacrophages play important roles in adipose tissue inflammation and its consequences. Unfortunately, a detailed description of the macrophage phenotypes in different human adipose tissues is not available.Subjects and methodsSubcutaneous, visceral and perivascular adipose tissues were obtained from 52 living kidney donors during live donor nephrectomy. Stromal vascular fractions were isolated, and the macrophage phenotypes were analyzed by flow cytometry using surface markers (CD14, CD16, CD36, and CD163).ResultsIn addition to CD16 positivity, pro-inflammatory macrophages also display high scavenger receptor CD36 expression. The great majority of CD16 negative macrophages express the anti-inflammatory CD163 marker. The presence of pro-inflammatory macrophages was almost twice as high in visceral (p < 0.0001) and perivascular (p < 0.0001) adipose tissues than in subcutaneous tissue. This difference was substantially more pronounced in the postmenopausal women subgroup, consequentlly, the total difference was driven by this subgroup.ConclusionWe obtained detailed information about M1 and M2 macrophage phenotypes in human adipose tissue. The visceral and perivascular adipose tissues had substantially higher pro-inflammatory characteristics than the subcutaneous tissue. The higher proportion of pro-inflammatory macrophages in the visceral adipose tissue of postmenopausal women might be related to an increased cardiovascular risk.

【 授权许可】

CC BY   
© The Author(s) 2016